©-glucosidase system of the thermophilic fungus Chaetomium thermophile

by Aldons Jekabs Lusis

Written in English
Published: Pages: 92 Downloads: 855
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Subjects:

  • Thermophilic fungi.

Edition Notes

Statementby Aldons Jekabs Lusis.
The Physical Object
Pagination[11], 92 leaves, bound :
Number of Pages92
ID Numbers
Open LibraryOL14239808M

Erisken, J. and Goksoyr, J. () The effect of temperature on growth and cellulase (B-1–4 endoglucanase) production in the compost fungus Chaetomium thermophile var. dissitum, Arch. . While in search of an enzyme for the conversion of xylose to xylitol at elevated temperatures, a xylose reductase (XR) gene was identified in the genome of the thermophilic fungus Chaetomium .   The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile n. var. Lusis AJ, Becker RR. Biochim Biophys Acta, (1), 01 Nov Cited by . Fungal strains are known to be efficient β-glucosidase producers; for instance Trichoderma and Aspergillus sp. Thermophilic fungi (Chaetomium thermophilum, Humicola insolens, Sporotrichum thermophile .

Cloning of a gene encoding thermostable cellobiohydrolase from the thermophilic fungus Chaetomium thermophilum and its expression in Pichia pastoris. which is then cleaved by β‐glucosidase to glucose It has been reported that the thermophilic fungus . trichum thermophile and Talaromyces leycettanus (five each), while only The B-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile n. var. Bio-chem. Biophys. . Murray P, Aro N, Collins C, Penttilä M, Saloheimo M, Tuohy M: Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus . Edelman, G., Ginsburg, V., and Hassid, W. Z., J. Biol. Chem., , ().Brown, S. A., and Neish, A. C., Canad.J. Biochem. Physiol., 32, ().Neish, A.

  Chaetomium thermophilum is a thermophilic fungus living in the high temperature environment up to 60degC belonged to the phylum Ascomycota. By now, many glycoside hydrolases have been isolated from C. thermophilum, such as a GH55 b-1,3-glucanase (Papageorgiou and Li, ), a b-glucosidase .

©-glucosidase system of the thermophilic fungus Chaetomium thermophile by Aldons Jekabs Lusis Download PDF EPUB FB2

The thermophilic fungus Chaetomium thermophile was shown to contain three major p-glucosidases, a cell-bound cellobiase, a cell- bound aryl-p-glucosidase and an extracellular aryl-B-glucosidase. The thermophilic fungus Chaetomium thermophile was shown to contain three major β-glucosidases, a cell-bound cellobiase, a cellbound aryl-β-glucosidase and an extracellular aryl-β-glucosidase Author: Aldons Jekabs Lusis.

Chaetomium thermophile contains three distinct β-glucosidases. Two of the enzymes are cell bound while the third is extracellular. On ©-glucosidase system of the thermophilic fungus Chaetomium thermophile book basis of rel Cited by: Chaetomium thermophile contains three distinct β-glucosidases.

Two of the enzymes are cell bound while the third is extracellular. On the basis of relative substrate specificities toward p- nitrophenyl -β- d -glucoside and cellobiose, one of the cell-bound enzymes is classified as a cellobiase while the other two enzymes are classified as aryl-β- d by: Abstract Graduation date: The thermophilic fungus Chaetomium thermophile was shown to contain three major β-glucosidases, a cell-bound cellobiase, a cellbound aryl-β-glucosidase and an extracellular aryl-β-glucosidase.

Here, the crystal structure of a family GH3 β-glucosidase from the thermophilic fungus Chaetomium thermophilum (Ct BGL) was determined at a resolution of Å.

The structure showed the three-domain architecture found in other β-glucosidases. The thermophilic fungus Chaetomium thermophilum var. coprophilum produced large amounts of extracellular and intracellular beta-glucosidase activity when grown on cellulose or cellobiose as.

Thermophilic Chaetomium (Chaetomium thermophilum) is one of the most extreme eukaryotic thermophiles that has revolutionized the biotechnological production of versatile enzymes with. Lusis, A.D.

and Becker, R.R. () The β-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile N. var.

Biochim. Biophys. Acta5– PubMed. Laccase from culture filtrates of a thermophilic fungus reported as Chaetomium thermophilium was purified by ultrafiltration, anion-exchange chromatography, and affinity chromatography.

The enzyme was a glycoprotein of 77 kDa. Chaetomium thermophile contains three distinct β-glucosidases. Two of the enzymes are cell bound while the third is extracellular.

The aim of the present investigation was to study the /3-glucosidase system of Sporotrichum thermophile for its possible implications in cellulolysis.

As will be shown, no evidence was found for a mul- tiple /3-glucosidase system in this fungus. Abstract Multiple forms of beta-glucosidase (EC ) of Sporotrichum thermophile were produced when the fungus was grown in a cellulose medium. One beta-glucosidase was purified. (endo-1,4-D-glucanase,EC ) was produced from a thermostable fungus Chaetomium thermophile.

It was grownon Vogel's medium with different carbon sources like xylan. Lusis AJ, Becker RR. The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var.

coprophile n. var. Biochim Biophys Acta. Nov 2; (1):5– Macris BJ. Production and Characterization of Cellulase and beta-Glucosidase. Abstract. Thermophilic Chaetomium species were first isolated from environmental samples such as straw, horse dung, oil palm kernels, wood chips, and bird nests.

Thermophilic Chaetomium (Chaetomium thermophilum) is one of the most extreme eukaryotic thermophiles. The wild-type cbh2 gene of the thermophilic fungus Chaetomium thermophilum encoding CBHII was mutagenized through in vitro directed evolution (Wang et al., b). The resulting Pichia pastoris.

Overall structure of the catalytic α subunit of glucosidase II. Considering the potential protein stability, Chaetomium thermophilum, a thermophilic fungus, which survives at temperatures.

TABLE 1. Taxonomic status and cardinal temperatures of thermophilic fungia Fungus (present nomenclature) Other names T Opt (°C) T max (°C) Canariomyces thermophila Guarro & Samson 45 Chaetomium mesopotamicum Abdullah & Zora 45 52 Chaetomium thermophile.

Luis A J and Beaker R R The B-glucosidase system of thermophilic fungusChaetomium thermophile hile;Biochem. Biophys. Biophys. Acta 5–19 Google Scholar. β-Glucosidase from the fungusThermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps—ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized.

After the first anion exchange chromatography, β-glucosidase. Read the latest articles of Biochimica et Biophysica Acta (BBA) - General Subjects atElsevier’s leading platform of peer-reviewed scholarly literature.

Thermophilic fungi isolated from partially decomposed vegetation from the interior of a recently used alligator (Alligator mississipiensis) nest included Chaetomium thermophile var. coprophile. Research. Chaetomium thermophilum is a focus of research at the Biochemistry Department at Heidelberg University, fungi are eukaryotic and not distant from animals they are good models for comparative and easy-to-manipulate research, and in the case of C.

thermophilum, it is of specialgiven the fact it is thermophilic, proteins derived from this fungus. SUMMARY Thermophilic fungi are a small assemblage in mycota that have a minimum temperature of growth at or above 20°C and a maximum temperature of growth extending up to 60 to 62°C. As the only representatives of eukaryotic organisms that can grow at temperatures above 45°C, the thermophilic.

The thermophilic fungus Chaetomium thermophilum var. coprophilum produced large amounts of extracellular and intracellular β‐glucosidase activity when grown on cellulose or cellobiose as carbon sources.

The presence of glucose in the culture medium drastically decreased the level of β‐glucosidase. Maximal β-glucosidase activity was attained 8–10 h after inducer addition to exponential phase growing cultures.

The induction is depressed in the presence of glucose. The system is composed of two electrophoretically different β-glucosidase. cellulolytic rates of some thermophilic fungi (Chaetomium thermophile, Sporotrichum thermophile, and Thermoascus aurantiacus) were two or three times that of Trichoderma viride, one of the most cellulolytic mesophilic fungi (15).

Romanelliet al. (20) reported that, ofthe three thermophilic fungi, S. thermophile. Lusis AJ, Becker RR. The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var.

coprophile n. var. Biochim Biophys Acta. Nov 2; (1):5– Roth WW, Srinivasan VR. Affinity chromatographic purification of beta-glucosidase. "This book aims to fill the gap by documenting thermophilic fungi discovered over the past five decades.

The chapters spans from covering basic aspects, taxonomy and classification including molecular phyologeny and biotechnological applications of thermophilic.

A printed edition of this Special Issue is available at MDPI Books. share announcement. Need Help? Support. Find support for a specific problem on the support section of our website. Get Support .Lusis AJ, Becker RR.

The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile n. var. Biochim Biophys Acta. Nov 2; (1):5– MANDELS M. Aims: Chaetomium thermophilum is a soil‐borne thermophilic fungus whose molecular biology is poorly understood. Only a few genes have been cloned from the Chaetomium genus.

This .