Spectroscopic membrane probes



Publisher: CRC Press in Boca Raton, Fla

Written in English
Published: Downloads: 368
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Subjects:

  • Cell membranes.,
  • Spectrum analysis -- Technique.,
  • Cell Membrane -- physiology.,
  • Cell Membrane -- ultrastructure.,
  • Electron Probe Microanalysis -- methods.,
  • Spectrometry, Fluorescence -- methods.,
  • Spectrum Analysis -- methods.

Edition Notes

Includes bibliographies and indexes.

Statementeditor, Leslie M. Loew.
ContributionsLoew, Leslie M.
Classifications
LC ClassificationsQH601 .S64 1988
The Physical Object
Pagination3 v. :
ID Numbers
Open LibraryOL2389065M
ISBN 100849345340, 0849345359
LC Control Number87018254

The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. Using fluorescence spectroscopic methods, we compared the membrane properties of intact fibroblasts from both normal subjects and patients with Huntington disease (HD). Cells were stained with various fluorophores, including 1-anilinonaphthalene sulfonic acid (ANS), 2-toluidinylnaphthalene sulfonic acid (TNS), 1,6-diphenyl-1,3,5. Spectroscopy NMR, IR, MS, UV-Vis Main points of the chapter 1. Hydrogen Nuclear Magnetic Resonance a. Splitting or coupling (what’s next to what) b. Chemical shifts (what type is it) c. Integration (how many are there) 2. 13C NMR 3. InfraRed spectroscopy (identifying functional groups) 4.   Magnetic resonance spectroscopic and imaging techniques are methods of choice for in vivo applications in animals and humans due to sufficient depth of microwave penetration in living tissues. Nuclear magnetic resonance, NMR, and its imaging modality, MRI, have found numerous biomedical and clinical applications but still suffer from limited functional resolution.

The main objective of the Membrane Protein Structural Dynamics consortium is to obtain a mechanistic understanding of how the structural dynamics of membrane proteins generate its function. The experimental study of structural dynamics is approached with a variety of spectroscopic methods and biophysical procedures that are in constant. We have investigated the deuterium (2 H) nuclear magnetic resonance (NMR) spectra of two 2 H-labeled fluorescence probes (trans,trans,trans-1,6-diphenylhexa-1,3,5-trienes, DPHs) incorporated into model lipid bilayer membrane systems at various temperatures. The membranes consisted of multilamellar bilayers of 1,2-dimyristoyl-sn-glycerophosphocholine (DMPC) containing varying concentrations. Detection of motional heterogeneities in lipid bilayer membranes by dual probe fluorescence correlation spectroscopy Jonas Korlacha, Tobias Baumgarta, Watt W. Webba, Gerald W. Feigensonb,* aSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY , USA bField of Biophysics, Cornell University, Ithaca, NY , USA Received 10 July ; received in revised form Deuterated phospholipids as Raman spectroscopic probes of membrane structure. Phase diagrams for the dipalmitoyl phosphatidylcholine(and its d62 derivative)-dipalmitoyl phosphatidylethanolamine system.

Kim and her group propose to do this with a powerful suite of spectroscopic tools, including steady state and time-resolved resonance emission spectroscopy (fluorescence, phosphorescence, and UV resonance Raman) along with fluorescence correlation spectroscopy. Membrane proteins are believed to comprise roughly 30% of all cellular proteins, and. Spectroscopy is the study of the interaction between matter and electromagnetic radiation as a function of the wavelength or frequency of the radiation. Historically, spectroscopy originated as the study of the wavelength dependence of the absorption by gas phase matter of visible light dispersed by a can also consider matter waves and acoustic waves as forms of radiative energy, and.

Spectroscopic membrane probes Download PDF EPUB FB2

Spectroscopic Membrane Probe 1st Edition by Leslie M. Loew (Author) ISBN ISBN Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book. The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane.

The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience.

The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe.

Additional Physical Format: Online version: Spectroscopic membrane probes. Boca Raton, Fla.: CRC Press, © (OCoLC) Document Type: Book. Get this from a library. Spectroscopic membrane probes.

Volume 2. [Leslie M Loew;] -- The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the. Spectroscopic membrane probes. Volume 1 | Loew, Leslie M | download | B–OK.

Download books for free. Find books. Article Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria Lindsey N. Miller,1 William T. Brewer,2 Julia D. Williams,2 Elizabeth M. Fozo,2 and Tessa R. Calhoun1,* 1Department of Chemistry and 2Department of Microbiology, University of Tennesseee, Knoxville, Tennessee ABSTRACT.

Title: Overview of Common Spectroscopic Methods to Determine the Orientation/Alignment of Membrane Probes and Drugs in Lipidic Bilayers VOLUME: 9 ISSUE: 9 Author(s):Silvia C.

Lopes and Miguel A. Castanho Affiliation:Centro de Quimica e Bioquimica da Faculdade de Ciencias da Universidade de Lisboa, Campo Grande, Ed.C8, Lisboa, Portugal.

Purchase Probes and Membrane Function - 1st Edition. Print Book & E-Book. ISBN  Pyrene probes. Pyrene (Fig. 1b) is a polycyclic aromatic hydrocarbon notable for its spectroscopic properties, namely the unusually long fluorescence lifetime (> ns in a variety of solvents and membrane systems) and ability to form to these distinctive features, both free pyrene and pyrene-labeled lipids have been used in membrane biophysics studies (Somerharju ).

This was amply demonstrated at the 8th Conference on Methods and Applications of Fluorescence: Probes,Imaging,and Spectroscopy held in Prague,the Czech Republic on August 24th 28th, This formed a crossroad of ideas from a variety of natural science and technical research fields and biomedical applications in : Hardcover.

Abstract. Raman spectroscopy is a valuable tool for qualitative and quantitative polymeric membrane characterization. The interactions among the functional groups, chain orientation, structural changes upon treatment/modification, and interfacial properties of the polymeric composite are some of the interesting information that can be revealed from the Raman spectra.

Fluorescent probes have been employed for more than half a century to study the structure and dynamics of model and biological membranes, using spectroscopic and/or microscopic experimental approaches. While their utilization has led to tremendous progress in our knowledge of membrane biophysics and physiology, in some respects the behavior of bilayer-inserted membrane probes has.

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results.

Book chapters by staff and students of the LFD Laboratory for Fluorescence Dynamics Assessment of membrane fluidity in individual yeast cells by Laurdan Generalized Polarization and multi-photon scanning fluorescence microscopy. Fluorescence Spectroscopy, Imaging and Probes (Vol. By R Kraayenhof, AJWG Visser, and HC Gerritsen (Editors).

The most complete fluorescent labeling and detection reference available, The Molecular Probes Handbook—A Guide to Fluorescent Probes and Labeling Technologies contains over 3, reagents and kits representing a wide range of Invitrogen Molecular Probes labeling and detection products.

The significantly revised 11th edition features extensive references, reorganized content, and new. Interaction of human serum albumin with liposomes of saturated and unsaturated lipids with different phase transition temperatures: a spectroscopic investigation by membrane probe PRODAN R.

Thakur, A. Das and A. Chakraborty, RSC Adv.,4,   Dense homogeneous PPO membranes were prepared by casting a solution, which consisted of PPO, TEMPO (spin probe), and 1,1,2-trichloroethylene solvent.

The solvent was evaporated at 22, 4, and −10°C. Membranes were subjected to EPR spectroscopic study as well as to the permeability measurement of various gases, including O 2, N 2, CO 2, and CH. To understand the chemistry of membranes, ideally one needs access to multiple length scales simultaneously, using probes that are non-invasive, label-free and membrane-interface specific.

ESR Spectroscopy in Membrane Biophysics Dr. Marcus A. Hemminga, Wageningen University Dr. Lawrence J. Berliner, University of Denver Membrane proteins offer the greatest challenge in structural biology, and there is an urgent need to develop and apply new biophysical methodologies that are able to generate detailed structural information.

Techniques include: Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red.

An alternative approach involves the use of extrinsic “probes”. These are small molecules which are introduced into the system, preferably at specific “sites”, and can serve as a “handle”, again mainly through their spectroscopic properties, for following biochemical events associated with specific regions of membranes.

Overview of Common Spectroscopic Methods to Determine the Orientation/Alignment of Membrane Probes and Drugs in Lipidic Bilayers Article (PDF Available) in Current Organic Chemistry 9(9) June. Here the use of phosphorescence (triplet) probes for measuring the rotational diffusion of intrinsic and extrinsic membrane proteins will be described.

Besides yielding information on internal fluctuations within the protein, this technique enables the measurement of comparatively slow rotational diffusion (> Pa s = 1 cp), i.e., in. Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level† M.

Chighizola, a A. Previdi, a T. Dini, a C. Piazzoni, a C. Lenardi, a P. Milani, a C. Schulte * a and A. Podestà * a. This higher membrane affinity for FM is not surprisinggiven that FM has a longer conjugationlength than FM (Fig.

2), making it a more lipophilicmoleculefor strongeradsorptionto the ing betweenbacterialspecies,the. faecalis. membraneshowshigher affinity for both discussedbelow,this may arise. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry.

These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. Stereoselective Total Synthesis of Dihydrocorynantheol; Discovery of (S)(3-Cyclopentyl(4-(trifluoromethyl)-1H-imidazolyl)propanamido)nicotinic Acid as a Hepatoselective Glucokinase Activator Clinical Candidate for Treating Type 2 Diabetes Mellitus.

Solid-state NMR (ssNMR) spectroscopy is a special type of nuclear magnetic resonance (NMR) spectroscopy, characterized by the presence of anisotropic (directionally dependent) interactions. Compared to the more common solution NMR spectroscopy, ssNMR usually requires additional hardware for high-power radio-frequency irradiation and magic-angle spinning.

The last 10 years have seen an enormous growth in our understanding of the molecular organisation of biological membranes. Experimental methods have been devised to meas­ ure the translational and rotational mobility of lipids and proteins, thereby furnishing a quantitative basis for the concept of.

Applications of Fluorescence Lifetime Spectroscopy and Imaging to Lipid Domains In Vivo 1. The Challenge of Studying Lipid Domains In Vivo Defied by Fluorescence Lifetimes. 2. Importance of Studies In Vitro to Design and Rationalize Studies In Vivo.

3. Labeling Cell Membranes In Vivo: Lipophilic Probes Versus GFP-Tagged Membrane Proteins. 4.Spin Probes. A spin probe is molecule with an unpaired electron that can associated to another molecule and can be used with electron paramagnetic resonance (EPR) spectroscopy to identify lipid membrane dynamics.

Common species include nitroxide radicals associated with either the hydrophilic headgroup or the hydrophobic chains of the lipid molecules that can then be identified by EPR to.When bound to the membrane the dye also exhibits concentration-dependent fluorescence changes.

The potential-sensitive response of the dye in Na(+),K(+)-ATPase membrane fragments after addition of MgATP in the presence of Na+ ions cannot be explained by a purely electrochromic mechanism.